TY  - SER
AU  - Vásquez Trujillo,Adolfo
TI  - Detection of Leishmania SPP, in Canines and Species of The Genus Lutzomyia in a Rural Area of Villavicencio, Venezuela
SN  - NJ08054
CY  - [Place of publication not identified]
PB  - AMCA
KW  - PATOLOGIA VETERINARIA - PUBLICACIONES SERIADAS
KW  - LEISMANIASIS - CONTROL - PUBLICACIONES SERIADAS
KW  - CANINOS - ENFERMEDADES - PUBLICACIONES SERIADAS
N2  - In 2005, before the apperance of new cases of cutaneous leishmaniasis in humana and canines in rural area of Villavicencio village The Reforma (Meta), we conducted a study to detect leishmania infections in dogs and lutzomyia present in the area using PCR. We scraped active lesions in 6 dogs for direct diagnosis and collected insects in domiciliary y area and in the forest around 40 houses using CDC light traps between 1800 and 2200 h. We processed samples from the 6 canines and 181 pools of sand flies (1-3 females per pool), for the PCR, we used universal primers for genus leishminia OLI and OL2, for subgenera B1 and B2 and species- specific sequences. A dog sample was positve for leishmania (subgenus viannia) guyanensis
ER  -