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  <titleInfo>
    <title>Detection of Leishmania SPP, in Canines and Species of The Genus Lutzomyia in a Rural Area of Villavicencio, Venezuela</title>
  </titleInfo>
  <name type="personal">
    <namePart>Vásquez Trujillo, Adolfo</namePart>
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      <roleTerm authority="marcrelator" type="text">creator</roleTerm>
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  </name>
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    <issuance>continuing</issuance>
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    <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
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  <abstract>In 2005, before the apperance of new cases of cutaneous leishmaniasis in humana and canines in rural area of Villavicencio village The Reforma (Meta), we conducted a study to detect leishmania infections in dogs and lutzomyia present in the area using PCR. We scraped active lesions in 6 dogs for direct diagnosis and collected insects in domiciliary y area and in the forest around 40 houses using CDC light traps between 1800 and 2200 h. We processed samples from the 6 canines and 181 pools of sand flies (1-3 females per pool), for the PCR, we used universal primers for genus leishminia OLI and OL2, for subgenera B1 and B2 and species- specific sequences. A dog sample was positve for leishmania (subgenus viannia) guyanensis.</abstract>
  <note type="statement of responsibility">Adolfo Vásquez Trujillo.</note>
  <subject>
    <geographicCode authority="marcgac">xxu</geographicCode>
  </subject>
  <subject authority="">
    <topic>PATOLOGIA VETERINARIA - PUBLICACIONES SERIADAS</topic>
  </subject>
  <subject authority="">
    <topic>LEISMANIASIS - CONTROL - PUBLICACIONES SERIADAS</topic>
  </subject>
  <subject authority="">
    <topic>CANINOS - ENFERMEDADES - PUBLICACIONES SERIADAS</topic>
  </subject>
  <relatedItem type="host">
    <titleInfo>
      <title>American Mosquito Control Association. AMCA</title>
    </titleInfo>
    <originInfo>
      <publisher>Florida : AMCA</publisher>
    </originInfo>
    <identifier type="issn">NJ08054</identifier>
    <part>
      <text>No.1-5, (2007). -- p. 42</text>
    </part>
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  <identifier type="issn">NJ08054</identifier>
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    <recordCreationDate encoding="marc">150107</recordCreationDate>
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    <recordIdentifier>030265</recordIdentifier>
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      <languageTerm authority="iso639-2b" type="code">spa</languageTerm>
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