01686nab a2200253 i 450000100070000000500170000700700030002400800410002702200120006804000230008004100080010304300080011110000310011924501530015026400500030333600270035333700330038033800280041352007370044165000510117865000520122965000520128177300990133303026520251030133023.0t|150107s0000####xx##################eng#d  aNJ08054  aCO-ViULLerdabspa0 aeng  axxu1 aVásquez Trujillo, Adolfo10aDetection of Leishmania SPP, in Canines and Species of The Genus Lutzomyia in a Rural Area of Villavicencio, Venezuela /cAdolfo Vásquez Trujillo. 1a[Place of publication not identified] :bAMCA  atextobtxt2rdacontent  asin mediaciónbn2rdamedia  avolumenbnc2rdacarrier  aIn 2005, before the apperance of new cases of cutaneous leishmaniasis in humana and canines in rural area of Villavicencio village The Reforma (Meta), we conducted a study to detect leishmania infections in dogs and lutzomyia present in the area using PCR. We scraped active lesions in 6 dogs for direct diagnosis and collected insects in domiciliary y area and in the forest around 40 houses using CDC light traps between 1800 and 2200 h. We processed samples from the 6 canines and 181 pools of sand flies (1-3 females per pool), for the PCR, we used universal primers for genus leishminia OLI and OL2, for subgenera B1 and B2 and species- specific sequences. A dog sample was positve for leishmania (subgenus viannia) guyanensis.07aPATOLOGIA VETERINARIA - PUBLICACIONES SERIADAS07aLEISMANIASIS - CONTROL - PUBLICACIONES SERIADAS07aCANINOS - ENFERMEDADES - PUBLICACIONES SERIADAS0 tAmerican Mosquito Control Association. AMCAdFlorida : AMCAgNo.1-5, (2007). -- p. 42xNJ08054